Block

5% NDS in 1xPBS containing ​

• 0.6% Triton

• 25% dimethyl sulfoxide

• 0.01% azide

(overnight, RT)

Primary

​5% NDS in 1xPBS containing 

• 0.8% Triton

• 5% DMSO

• 1:2000 Rabbit anti-NF

• 1:100 Mouse anti-MBP

(5 days, RT)

Secondary

​5% NDS in 1xPBS containing 

• 0.8 % Triton

• 5% DMSO

• 1:150 donkey anti-rabbit (488 or 568)

• 1:150 donkey anti-mouse (488 or 568)

• 1:1000 DAPI 

(7 days, RT)

Index Matching

• 67% thiodiethanol in water